| Primary Identifier | MGI:8435605 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Inserted expressed sequence | Gene | Sptan1 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false | Project Collection | CPMM |
| molecularNote | Using CRISPR/Cas9 editing in mouse embryos, exons 53, 54, 55, 56, and part of exon 57 in Sptan1 were replaced with corresponding SPTAN1 human (Hs) exons 53-57 containing the c.6908_6916dup (p.Asp2303_Leu2305dup) variant. Exon 53 was inserted in the antisense orientation, additionally a Lox71 site was inserted upstream of the antisense human exon 53 and a reverse complement Lox66 site was inserted downstream of the antisense human exon 53. Prior to cre-mediated recombination, the allele functions as a knock-out first with splicing from exons 52 to 54 creating a premature stop. With cre-mediated recombination, the antisense exon containing the variant are rotated into the sense orientation for expression of the variant-containing humanized region. |
