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Allele : Gt(ROSA)26Sor<tm1(CAG-Mlxipl*)Mher> gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mark A Herman
Primary Identifier  MGI:7428861 Allele Type  Targeted
Attribute String  Conditional ready, Epitope tag, Inserted expressed sequence, No functional change Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  (129S6/SvEvTac x C57BL/6NCrl)F1
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting construct inserted into the locus an initial FRT site, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), 3xFlag-tagged truncated Mxlipl cDNA (coding the ChREBPbeta isoform but not the full-length 3ChREBP alpha isoform), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine growth hormone polyadenylation (bGHpA) signal and a second FRT site. The construct also contains an attB/attP-flanked neomycin cassette. This targeting vector was inserted into intron 1 of the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • 3xFlag-tagged ChREBPbeta,
  • Gt(ROSA)26Sor<tm1(CAG-Mlxipl)Dks>,
  • Gt(ROSA)26Sor<tm1(CAG-Mlxipl)Dks>,
  • 3xFlag-tagged ChREBPbeta
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