| Primary Identifier | MGI:7523863 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Gt(ROSA)26Sortm57(CAG-hop/EGFP)Hze> line described in J:219930 was crosed to cre deleter to remove the LSL cassette leaving the CAG-FSF-Jaws-GFP-ER2-WPRE-bGHpA inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The hop/EGFP fusion protein (Halo57*K200R*W214F/EGFP or Jaws) is composed of a modified Halobacterium halobium (strain shark)-derived halorhodopsin (Halo57) fused in-frame via a 15 nucleotide linker to the N-terminus of enhanced green fluorescent protein (EGFP). The Halo57 sequence has K200R and W214F mutations to substantially boost photocurrents. An endoplasmic reticulum exporting sequence (ER2) from the Kir2.1 inward rectifier potassium channel gene (amino acids FCYENEV) at the C-terminal of EGFP improves membrane trafficking/prevents aggregation. It is a Flp-dependent reporter with an optogenetic silencer function. |
