| Primary Identifier | MGI:7865614 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Lrrk2 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The allele was designed to incorporate a conditional R1441G point mutation into exon 31 of the leucine-rich repeat kinase 2 (Lrrk2) gene. The targeting vector, which was inserted into the endogenous Lrrk2 intron 30, consists of an initial loxP site, followed by an FRT-flanked neomycin cassette, exons31-51 Lrrk2 cDNA including the 3' untranslated region (UTR), an hGHpA (2.1 kb of the human growth hormone gene harboring the hGH polyadenylation signal), an F3-flanked puromycin cassette, and a second loxP site. Genomic exon 31 harboring the R1441G mutation is located immediately downstream of the second loxP site. FLP-recombinase removed the FRT-flanked neomycin cassette and the F3-flanked puromycin cassette. |
