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Allele : Gt(ROSA)26Sor<em1(CAG-Trim21)Lmeh> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Lisa Mehlmann
Primary Identifier  MGI:8163997 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  The allele was generated by cloning an HA-Trim21 sequence into the GT(ROSA)26Sor locus (Ai9, Addgene 22799), replacing the tdTomato sequence. The final targeting construct consists of a CAG (CMV enhancer/chicken beta-actin) promoter, a loxP-flanked 3xSV40 polyadenylation (STOP) signal, an HA-tagged tripartite motif-containing 21 (Trim21) sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and a BGH polyA signal.
  • mutations:
  • Insertion
  • synonyms:
  • HA-Trim21,
  • HA-Trim21
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0 Mutation Involves

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