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Allele : Gt(ROSA)26Sor<em3(CAG-TFRC/OVAL)Dest> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 3, Daria Esterhazy
Primary Identifier  MGI:8283244 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Epitope tag, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  The allele was generated by CRISPR/Cas9 endonuclease-mediated genome editing using crRNAs (ACTCCAGTCTTTCTAGAAGA) and tracrRNAs to insert a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked Neo/STOP cassette, residues 1-118 of the human transferrin receptor (TFRC) fused to residues 139-386 of the chicken ovalbumin gene (OVAL), a 3XFLAG tag, and a bovine growth hormone polyadenylation signal (bGHpolyA) into the Gt(ROSA)26Sor locus on Chromosome 6.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26-tmO,
  • Rosa26-tmO
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