| Primary Identifier | MGI:8394039 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S/SvEv x C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Embryonic stem cells carrying the Igk mutant Igktm2.1Fwa allele were re-targeted to introduce a CAG-hTdT cassette to intron 1 of the mouse Gt(ROSA)26Sor gene, downstream of the Igk allele on Chromosome 6. Expression of the human terminal deoxynucleotidyl transferase (hTdT; formal gene symbol DNTT, DNA nucleotidylexotransferase) cDNA is driven by the CAG promotor during light chain rearrangement in pre-B cells. It adds random nucleotides to VH-D and D-JH junctions to form N regions, and promotes human-like diversification of the light chain cassette. |
