Protein Gawky (also known as GW182) plays a role in RNA-mediated gene silencing by micro-RNAs (miRNAs) [, ]. It is a component of cytoplasmic mRNA processing bodies required for early Drosophila development []. GW182 interacts with AGO1, and this interaction is essential for silencing by miRNAs []. It recruits the CCR4-NOT deadenylase complex through the interaction with PABPC1 [].
This entry represents the UBA domain of Gawky.Protein Gawky (also known as GW182) plays a role in RNA-mediated gene silencing by micro-RNAs (miRNAs) [, ]. It is a component of cytoplasmic mRNA processing bodies required for early Drosophila development []. GW182 interacts with AGO1, and this interaction is essential for silencing by miRNAs []. It recruits the CCR4-NOT deadenylase complex through the interaction with PABPC1 [].
BTG2 is a member of the BTG/Tob family. It plays an important role in cellular differentiation and cancer. It is a direct transcriptional target of p53 and has been shown to be a major effector of suppression of Ras-induced transformation by p53 []. BTG2 has been shown to function as a transcription cofactor []. BTG2 is also found to interact directly with the first RRM domain of the poly(A)-binding protein PABPC1 and promoted mRNA poly(A) tail shortening []. In mice, its overexpression during neurogenesis led to microcephaly, while its deletion triggered impaired neuronal differentiation [].The BTG/Tob family members contain an conserved N-terminal domain, known as APRO domain (AntiPROliferative) or BTG domain. In mammals, six family members have been identified: BTG1, BTG2/PC3/Tis21, BTG3/ANA, BTG4/PC3B, Tob1/Tob and Tob2. They interact with CAF1, a subunit of the CCR4-NOT complex. They display antiproliferative properties as their ectopic expression in a variety of cell lines reduced cell proliferation [].
