| Primary Identifier | MGI:5475220 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd70 |
| Transmission | Germline | Strain of Origin | 129 x C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neo cassette was inserted upstream of exon 1. The neo cassette was then excised in properly targeted cells by cre recombinase. A cassette composed of an FRT flanked neo and another loxP site was then inserted downstream of exon 2. This second neo cassette was then removed by crossing to mice carrying Tg(ACTFLPe)9205 Dym. Subsequently, mice were crossed to Tg(ACTB-cre)2Mrt mice to excise exons 1 and 2. Bone marrow derived dendritic cells stimulated with lipopolysaccharide fail to produce mutant mRNA and no protein is expressed on the surface of stimulated splenocytes. |
