Primary Identifier | MGI:6719079 | Allele Type | Targeted |
Attribute String | Conditional ready, Epitope tag, Reporter | Gene | Ascl2 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The coding region containing all 3 exons was replaced with a loxP-flanked Ascl2 cDNA with a 5' triple-FLAG epitope and a 3' mCherry cDNA separated from Ascl2 by a P2A cleavage sequence. An frt-flanked neomycin selection cassette was also inserted before the second loxP site and removed via Flp-mediated recombination. |