Primary Identifier | MGI:4889046 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gnao1 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An IRES-beta galactosidase and PGK-neomycin cassette was inserted in exon 7.1 at the aspartic acid position 262. Isoform Go1-alpha was disrupted while beta galactosidase was placed under the control of the Gnao1 promoter. Absence of the protein product from brain and pancreatic islets was confirmed immunologically. |