| Primary Identifier | MGI:2429646 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hsf2 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 200 bp exon containing the translational start codon and a 5' portion of the DNA binding domain was replaced with a neomycin selection cassette. Transcript was undetected by Northern blot analysis of brain, heart, kidney, liver, and lung tissue. Western blot analysis of testis extract indicated a lack of protein in homozygous mutant mice. To confirm that this absence was due to a disrupted allele and not the labile nature of the protein, MEFs treated with proteasome inhibitors were analyzed and similar results were obtained. |
