First Author | Tong P | Year | 2017 |
Journal | Proc Natl Acad Sci U S A | Volume | 114 |
Issue | 40 | Pages | E8411-E8420 |
PubMed ID | 28923960 | Mgi Jnum | J:252910 |
Mgi Id | MGI:6095409 | Doi | 10.1073/pnas.1704962114 |
Citation | Tong P, et al. (2017) IgH isotype-specific B cell receptor expression influences B cell fate. Proc Natl Acad Sci U S A 114(40):E8411-E8420 |
abstractText | Ig heavy chain (IgH) isotypes (e.g., IgM, IgG, and IgE) are generated as secreted/soluble antibodies (sIg) or as membrane-bound (mIg) B cell receptors (BCRs) through alternative RNA splicing. IgH isotype dictates soluble antibody function, but how mIg isotype influences B cell behavior is not well defined. We examined IgH isotype-specific BCR function by analyzing naturally switched B cells from wild-type mice, as well as by engineering polyclonal Ighgamma1/gamma1 and Ighepsilon/epsilon mice, which initially produce IgG1 or IgE from their respective native genomic configurations. We found that B cells from wild-type mice, as well as Ighgamma1/gamma1 and Ighepsilon/epsilon mice, produce transcripts that generate IgM, IgG1, and IgE in an alternative splice form bias hierarchy, regardless of cell stage. In this regard, we found that mIgmu > mIggamma1 > mIgepsilon, and that these BCR expression differences influence respective developmental fitness. Restrained B cell development from Ighgamma1/gamma1 and Ighepsilon/epsilon mice was proportional to sIg/mIg ratios and was rescued by enforced expression of the respective mIgs. In addition, artificially enhancing BCR signal strength permitted IgE(+) memory B cells-which essentially do not exist under normal conditions-to provide long-lived memory function, suggesting that quantitative BCR signal weakness contributes to restraint of IgE B cell responses. Our results indicate that IgH isotype-specific mIg/BCR dosage may play a larger role in B cell fate than previously anticipated. |