Primary Identifier | MGI:7437637 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Lifr |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed hygro cassette was inserted upstream of exon 2 and a floxed neo cassette was inserted in the intron after exon 14. Successfully recombined ES cell clones were subjected to transient expression of Cre recombinase by transfection of pMC1-Cre. The resulting clone 18 with exons 2 to 14 floxed was used for the germline transmission. |