| First Author | Kaushik A | Year | 1995 |
| Journal | Eur J Immunol | Volume | 25 |
| Issue | 2 | Pages | 631-4 |
| PubMed ID | 7875225 | Mgi Jnum | J:23271 |
| Mgi Id | MGI:71037 | Doi | 10.1002/eji.1830250249 |
| Citation | Kaushik A, et al. (1995) The nude mutation results in impaired primary antibody repertoire. Eur J Immunol 25(2):631-634 |
| abstractText | We have studied the effect of the nude mutation and/or T lymphocytes on the development of V gene germ-line repertoire in neonatal athymic (nu/nu) and euthymic (+/nu) littermates. A total of 2.35 x 10(6) and 1.47 x 10(6) B lymphocyte clones from nu/nu and +/nu neonates, respectively, were examined for the expression of select V- H (J558, J606, S107, 36-60, 7183 and Q52) and V-K (1, 2, 8 and 9) gene families as well as V-H (J558, S107) + V-K (1, 9) associations. Data showed that the nude mutation, whether homozygous or heterozygous, significantly affects V-H and V-K gene expression as well as V-H and V-K pairings and, thus, provide evidence for a defective development of B cell repertoire in both athymic nude (nu/ nu) and euthymic (+/nu) mice. In addition, an analysis of 3.34 x 10(6) B lymphocyte clones from adult C57BL/6 mice showed non-stochastic association between V(H)J558 + V(K)1 gene families that suggests restrictions on clonal population in order to maintain homeostasis in the immune system. Studies outlined here, therefore, describe an hitherto unknown defect in the development of B lymphocyte repertoire as a result of the nude mutation which is independent of thymic dysgenesis. |
