| First Author | Uapinyoying P | Year | 2023 |
| Journal | iScience | Volume | 26 |
| Issue | 8 | Pages | 107479 |
| PubMed ID | 37599828 | Mgi Jnum | J:339342 |
| Mgi Id | MGI:7521616 | Doi | 10.1016/j.isci.2023.107479 |
| Citation | Uapinyoying P, et al. (2023) Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle. iScience 26(8):107479 |
| abstractText | Fibro/adipogenic progenitors (FAPs) are skeletal muscle stromal cells that support regeneration of injured myofibers and their maintenance in healthy muscles. FAPs are related to mesenchymal stem cells (MSCs/MeSCs) found in other adult tissues, but there is poor understanding of the extent of similarity between these cells. Using single-cell RNA sequencing (scRNA-seq) datasets from multiple mouse tissues, we have performed comparative transcriptomic analysis. This identified remarkable transcriptional similarity between FAPs and MeSCs, confirmed the suitability of PDGFRalpha as a reporter for FAPs, and identified extracellular proteolysis as a new FAP function. Using PDGFRalpha as a cell surface marker, we isolated FAPs from healthy and dysferlinopathic mouse muscles and performed scRNA-seq analysis. This revealed decreased FAP-mediated Wnt signaling as a potential driver of FAP dysfunction in dysferlinopathic muscles. Analysis of FAPs in dysferlin- and dystrophin-deficient muscles identified a relationship between the nature of muscle pathology and alteration in FAP gene expression. |
