| Primary Identifier | MGI:3639095 | Allele Type | Gene trapped |
| Gene | Hsf1 | Inheritance Mode | Not Specified |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | Integration site GenBank ID: AB212666 |
| molecularNote | The randomly integrating (within a gene) gene trap vector pU18 contains 1.8 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by an internal ribosome entry site (IRES) and the Betageo beta-galactosidase/neomycin resistance fusion gene with a mammalian polyadenylation signal. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. Mutant and wild-type loxP sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. |
