Primary Identifier | MGI:6468283 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag | Gene | Piwil4 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Using an sgRNA targeting the start of the coding region and a DNA oligonucleotide template with CRISPR/Cas9 technology, HA epitope tag sequence was inserted immediately after the ATG start codon. This creates an allele that expresses a fully functional endogenous peptide with an N-terminal HA tag. |