| Primary Identifier | MGI:2182834 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Per1 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 5.3kb region, encompassing exons 4 through 18, was replaced by an Hprt minigene. The vector was designed such that the excision of the inserted cassette and potential subsequent splicing of exon 3 to exon 19 would introduce a frameshift mutation. Such a transcript was not found, however, a fusion transcript consisting of the first three exons of the endogenous gene aberrantly spliced to exons 3 through 9 of the Hprt transgene was identified by RT-PCR and sequence analysis. Because exons 1 through 3 encode a putatively non-functional region of the protein, the mutation was believed to be null. |
