| First Author | Schmid RM | Year | 1992 |
| Journal | Am J Physiol | Volume | 262 |
| Issue | 6 Pt 1 | Pages | G971-6 |
| PubMed ID | 1377451 | Mgi Jnum | J:1349 |
| Mgi Id | MGI:49876 | Doi | 10.1152/ajpgi.1992.262.6.G971 |
| Citation | Schmid RM, et al. (1992) Dietary regulation of pancreatic amylase in transgenic mice mediated by a 126-base pair DNA fragment. Am J Physiol 262(6 Pt 1):G971-6 |
| abstractText | Expression of the mouse pancreatic amylase gene Amy-2.2 is increased approximately 10-fold in response to increasing the carbohydrate content of the diet from 9.6 to 74%. The DNA sequence mediating this response has been localized to the 5' flanking region of the amylase gene by analysis of hybrid constructs in transgenic mice. The results define a 127-base pair dietary response unit that includes two previously described regulatory elements, an insulin-responsive element and a pancreatic enhancer. Fragments containing these two elements alone fail to respond to diet, demonstrating a requirement for additional regulatory sequences. Another mouse amylase gene Amy-2.1 is only minimally responsive to insulin and to diet. The data are consistent with the hypothesis that the insulin-response element is necessary but not sufficient for regulation of amylase by dietary carbohydrate. |
