| First Author | Mauvoisin D | Year | 2017 |
| Journal | Cell Rep | Volume | 20 |
| Issue | 7 | Pages | 1729-1743 |
| PubMed ID | 28813682 | Mgi Jnum | J:269829 |
| Mgi Id | MGI:6274029 | Doi | 10.1016/j.celrep.2017.07.065 |
| Citation | Mauvoisin D, et al. (2017) Circadian and Feeding Rhythms Orchestrate the Diurnal Liver Acetylome. Cell Rep 20(7):1729-1743 |
| abstractText | Lysine acetylation is involved in various biological processes and is considered a key reversible post-translational modification in the regulation of gene expression, enzyme activity, and subcellular localization. This post-translational modification is therefore highly relevant in the context of circadian biology, but its characterization on the proteome-wide scale and its circadian clock dependence are still poorly described. Here, we provide a comprehensive and rhythmic acetylome map of the mouse liver. Rhythmic acetylated proteins showed subcellular localization-specific phases that correlated with the related metabolites in the regulated pathways. Mitochondrial proteins were over-represented among the rhythmically acetylated proteins and were highly correlated with SIRT3-dependent deacetylation. SIRT3 activity being nicotinamide adenine dinucleotide (NAD)(+) level-dependent, we show that NAD(+) is orchestrated by both feeding rhythms and the circadian clock through the NAD(+) salvage pathway but also via the nicotinamide riboside pathway. Hence, the diurnal acetylome relies on a functional circadian clock and affects important diurnal metabolic pathways in the mouse liver. |
