| First Author | Bucheli OTM | Year | 2024 |
| Journal | Sci Rep | Volume | 14 |
| Issue | 1 | Pages | 8507 |
| PubMed ID | 38605071 | Mgi Jnum | J:357264 |
| Mgi Id | MGI:7622907 | Doi | 10.1038/s41598-024-58868-0 |
| Citation | Bucheli OTM, et al. (2024) Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis. Sci Rep 14(1):8507 |
| abstractText | While cellular metabolism was proposed to be a driving factor of the activation and differentiation of B cells and the function of the resulting antibody-secreting cells (ASCs), the study of correlations between cellular metabolism and functionalities has been difficult due to the absence of technologies enabling the parallel measurement. Herein, we performed single-cell transcriptomics and introduced a direct concurrent functional and metabolic flux quantitation of individual murine B cells. Our transcriptomic data identified lactate metabolism as dynamic in ASCs, but antibody secretion did not correlate with lactate secretion rates (LSRs). Instead, our study of all splenic B cells during an immune response linked increased lactate metabolism with acidic intracellular pH and the upregulation of apoptosis. T cell-dependent responses increased LSRs, and added TLR4 agonists affected the magnitude and boosted LSR(high) B cells in vivo, while resulting in only a few immunoglobulin-G secreting cells (IgG-SCs). Therefore, our observations indicated that LSR(high) cells were not differentiating into IgG-SCs, and were rather removed due to apoptosis. |
