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Publication : Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis.

First Author  Bucheli OTM Year  2024
Journal  Sci Rep Volume  14
Issue  1 Pages  8507
PubMed ID  38605071 Mgi Jnum  J:357264
Mgi Id  MGI:7622907 Doi  10.1038/s41598-024-58868-0
Citation  Bucheli OTM, et al. (2024) Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis. Sci Rep 14(1):8507
abstractText  While cellular metabolism was proposed to be a driving factor of the activation and differentiation of B cells and the function of the resulting antibody-secreting cells (ASCs), the study of correlations between cellular metabolism and functionalities has been difficult due to the absence of technologies enabling the parallel measurement. Herein, we performed single-cell transcriptomics and introduced a direct concurrent functional and metabolic flux quantitation of individual murine B cells. Our transcriptomic data identified lactate metabolism as dynamic in ASCs, but antibody secretion did not correlate with lactate secretion rates (LSRs). Instead, our study of all splenic B cells during an immune response linked increased lactate metabolism with acidic intracellular pH and the upregulation of apoptosis. T cell-dependent responses increased LSRs, and added TLR4 agonists affected the magnitude and boosted LSR(high) B cells in vivo, while resulting in only a few immunoglobulin-G secreting cells (IgG-SCs). Therefore, our observations indicated that LSR(high) cells were not differentiating into IgG-SCs, and were rather removed due to apoptosis.
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