First Author | Inoue K | Year | 1995 |
Journal | Brain Res Mol Brain Res | Volume | 28 |
Issue | 2 | Pages | 343-8 |
PubMed ID | 7723632 | Mgi Jnum | J:22673 |
Mgi Id | MGI:70531 | Doi | 10.1016/0169-328x(94)00244-9 |
Citation | Inoue K, et al. (1995) Cloning and expression of a bovine glutamate transporter. Brain Res Mol Brain Res 28(2):343-8 |
abstractText | We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake. |