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Publication : Cloning and expression of a bovine glutamate transporter.

First Author  Inoue K Year  1995
Journal  Brain Res Mol Brain Res Volume  28
Issue  2 Pages  343-8
PubMed ID  7723632 Mgi Jnum  J:22673
Mgi Id  MGI:70531 Doi  10.1016/0169-328x(94)00244-9
Citation  Inoue K, et al. (1995) Cloning and expression of a bovine glutamate transporter. Brain Res Mol Brain Res 28(2):343-8
abstractText  We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake.
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