| First Author | Kim J | Year | 2022 |
| Journal | Front Immunol | Volume | 13 |
| Pages | 1095140 | PubMed ID | 36741385 |
| Mgi Jnum | J:392721 | Mgi Id | MGI:7433487 |
| Doi | 10.3389/fimmu.2022.1095140 | Citation | Kim J, et al. (2022) Lysine methyltransferase Kmt2d regulates naive CD8(+) T cell activation-induced survival. Front Immunol 13:1095140 |
| abstractText | Lysine specific methyltransferase 2D (Kmt2d) catalyzes the mono-methylation of histone 3 lysine 4 (H3K4me1) and plays a critical role in regulatory T cell generation via modulating Foxp3 gene expression. Here we report a role of Kmt2d in naive CD8(+) T cell generation and survival. In the absence of Kmt2d, the number of CD8(+) T cells, particularly naive CD8(+) T cells (CD62L(hi)/CD44(lo)), in spleen was greatly decreased and in vitro activation-related death significantly increased from Kmt2d (fl/fl)CD4cre(+) (KO) compared to Kmt2d (fl/fl)CD4cre(-) (WT) mice. Furthermore, analyses by ChIPseq, RNAseq, and scRNAseq showed reduced H3K4me1 levels in enhancers and reduced expression of apoptosis-related genes in activated naive CD8(+) T cells in the absence of Kmt2d. Finally, we confirmed the activation-induced death of antigen-specific naive CD8(+) T cells in vivo in Kmt2d KO mice upon challenge with Listeria monocytogenes infection. These findings reveal that Kmt2d regulates activation-induced naive CD8(+) T cell survival via modulating H3K4me1 levels in enhancer regions of apoptosis and immune function-related genes. |
