| First Author | Wang X | Year | 2014 |
| Journal | Stroke | Volume | 45 |
| Issue | 10 | Pages | 3089-91 |
| PubMed ID | 25139875 | Mgi Jnum | J:382515 |
| Mgi Id | MGI:8314575 | Doi | 10.1161/STROKEAHA.114.006348 |
| Citation | Wang X, et al. (2014) Intervention of death-associated protein kinase 1-p53 interaction exerts the therapeutic effects against stroke. Stroke 45(10):3089-91 |
| abstractText | BACKGROUND AND PURPOSE: Death-associated protein kinase 1 (DAPK1) interacts with the tumor suppressor gene p53 via a direct binding of a death domain of DAPK1 to a DNA-binding motif (DM) of p53 (p53DM) and converges multiple cell death pathways in stroke. The goals of this study are to determine whether disruption of DAPK1-p53 interaction is therapeutically effective against stroke. METHODS: We synthesized a membrane-permeable p53DM peptide (Tat-p53DM) and tested the therapeutic effects of Tat-p53DM in a mouse model with stroke. RESULTS: We showed that Tat-p53DM blocked DAPK1-p53 interaction in brain cells in vivo. When administered 6 hours after stroke onset in adult male mice, Tat-p53DM was still therapeutically effective against brain damages and improved neurological functions. CONCLUSIONS: DAPK1-p53 interaction is a preferred target for therapeutic intervention of stroke. |
