| First Author | Yamabe Y | Year | 1999 |
| Journal | Gene | Volume | 227 |
| Issue | 1 | Pages | 39-47 |
| PubMed ID | 9931425 | Mgi Jnum | J:52878 |
| Mgi Id | MGI:1330542 | Doi | 10.1016/s0378-1119(98)00598-8 |
| Citation | Yamabe Y, et al. (1999) Structures of mouse Rep-8 cDNA and genomic clones. Gene 227(1):39-47 |
| abstractText | A mouse homologue of the human Rep-8 gene was cloned by PCR methods using degenerate oligonucleotide primers corresponding to highly conserved regions between human and mouse genes, and by the Marathon-Ready cDNA amplification method. The full-length mouse Rep-8 contains 1422 nucleotides and codes for a protein of 277 amino acids with a calculated mol. wt. of 31,519. The overall amino acid sequence homology between mouse and human Rep-8 proteins was 73%, and the overall nucleic acid sequence similarity was 65%. The predicted amino acid sequence of mouse Rep-8 has leucine zipper-like motifs in the N-terminal region, similar to the human Rep-8 protein. Rep-8 exists as a single-copy gene and is expressed in both the early and late embryonic stages of mouse development, suggesting that the Rep-8 gene product has an important role in embryogenesis. The genomic structure of the mouse Rep-8 gene was characterized extensively so that a gene targeting strategy could be used to develop an understanding of the biological function(s) of this interesting gene and its product. |
