| First Author | Rivera RR | Year | 1993 |
| Journal | Mol Cell Biol | Volume | 13 |
| Issue | 11 | Pages | 7163-9 |
| PubMed ID | 8413305 | Mgi Jnum | J:49827 |
| Mgi Id | MGI:1278306 | Doi | 10.1128/mcb.13.11.7163 |
| Citation | Rivera RR, et al. (1993) Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression. Mol Cell Biol 13(11):7163-9 |
| abstractText | We used a DNA-protein interaction screening method to isolate a cDNA, Erg-3, whose product binds to a site, designated pi, present in the immunoglobulin (Ig) heavy-chain gene enhancer. Erg-3 is an alternatively spliced product of the erg gene and contains an Ets DNA- binding domain. Fli-1 and PU.1, related Ets proteins, also bind to the same site. In addition, PU.1 binds to a second site, designated microB, in the Ig heavy-chain enhancer. We demonstrate that the pi binding site is crucial for Ig heavy-chain gene enhancer function. In addition, we show that Erg-3 and Fli.1, but not PU.1, can activate a reporter construct containing a multimer of protein-binding sites, synergistically with helix-loop-helix protein E12. We discuss how combinatorial interactions between members of the helix-loop-helix and Ets families may account for the tissue specificity of these proteins. |
