| First Author | Morgans CW | Year | 1993 |
| Journal | J Cell Sci | Volume | 106 ( Pt 4) |
| Pages | 1275-82 | PubMed ID | 8126106 |
| Mgi Jnum | J:16295 | Mgi Id | MGI:64379 |
| Doi | 10.1242/jcs.106.4.1275 | Citation | Morgans CW, et al. (1993) Generation of truncated brain AE3 isoforms by alternate mRNA processing. J Cell Sci 106(Pt 4):1275-82 |
| abstractText | AE3 gene is a member of the AE anion exchanger gene family that is expressed primarily in brain and heart. The principal product of the AE3 gene in rodent brain, FL-AE3p, when expressed in heterologous cell lines, gives rise to chloride-dependent changes in intracellular pH consistent with its operation as an anion exchanger. We have identified two novel isoforms of mouse AE3 that are generated by tissue-specific alternate RNA processing. One of these isoforms encodes a polypeptide, 14-AE3p, that corresponds to a portion of the NH2-terminal cytoplasmic domain of AE3. 14-AE3p lacks the entire transmembrane domain that-in FL-AE3p forms the anion exchange channel. Immunoblots with antibodies to the NH2- and COOH-termini confirm that FL-AE3 and 14-AE3 are expressed in rat brain as 160 kDa and 74 kDa polypeptides, respectively. Unlike FL-AE3p, however, 14-AE3p is insoluble in non-ionic detergent, suggesting a possible association with the cytoskeleton. |
