| First Author | Yorifuji T | Year | 1991 |
| Journal | Genomics | Volume | 10 |
| Issue | 3 | Pages | 547-50 |
| PubMed ID | 1716243 | Mgi Jnum | J:11413 |
| Mgi Id | MGI:59849 | Doi | 10.1016/0888-7543(91)90434-g |
| Citation | Yorifuji T, et al. (1991) Molecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator. Genomics 10(3):547-50 |
| abstractText | We have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common delta F508 mutation were conserved between human and mouse. |
