| First Author | Yanagimoto T | Year | 1994 |
| Journal | Biochim Biophys Acta | Volume | 1201 |
| Issue | 3 | Pages | 405-10 |
| PubMed ID | 7803471 | Mgi Jnum | J:22023 |
| Mgi Id | MGI:69917 | Doi | 10.1016/0304-4165(94)90069-8 |
| Citation | Yanagimoto T, et al. (1994) Molecular cloning and functional expression of a mouse cytochrome P-450 (Cyp3a-13): examination of Cyp3a-13 enzyme to activate aflatoxin B1 (AFB1). Biochim Biophys Acta 1201(3):405-10 |
| abstractText | A cDNA encoding a novel member of the cytochrome P-450 superfamily, Cyp3a-13, has been isolated from mouse liver cDNA library by hybridization screening. The Cyp3a-13 encoded 503 amino acid residues and shared 71% amino acid identity with Cyp3a-11. When Cyp3a-13 cDNA was expressed in CR119 cells which had been established as a cell line stably expressing NADPH-cytochrome P-450 reductase cDNA of guinea pigs, aflatoxin B1-dependent cytotoxicity was observed. This cytotoxicity was enhanced by alpha-naphthoflavone (7,8-benzoflavone), which is known to augment the CYP3A enzymatic activity. The results indicate that CYP3A in mice, which are relatively insensitive to aflatoxin B1, can activate aflatoxin B1 to a genotoxic product. |
