| First Author | Lafuse WP | Year | 1995 |
| Journal | J Leukoc Biol | Volume | 57 |
| Issue | 4 | Pages | 663-9 |
| PubMed ID | 7722423 | Mgi Jnum | J:24548 |
| Mgi Id | MGI:72286 | Doi | 10.1002/jlb.57.4.663 |
| Citation | Lafuse WP, et al. (1995) IFN-gamma increases cathepsin H mRNA levels in mouse macrophages. J Leukoc Biol 57(4):663-9 |
| abstractText | Expression of major histocompatibility complex (MHC) class II molecules and ability to present antigen to T lymphocytes is acquired upon activation of the macrophage by interferon-gamma (IFN-gamma). Little information is available concerning immune regulation of protease gene expression in mouse macrophages. We have isolated a cDNA clone for cathepsin H, a lysosomal cysteine proteinase from a cDNA subtraction library of mouse macrophage genes induced by IFN-gamma, and have characterized its expression. The level of cathepsin H mRNA increased in mouse peritoneal macrophages following addition of IFN-gamma. Cathepsin H mRNA levels began to increase 8 h after the addition of IFN-gamma and was maximal at 24-48 h. This increase was concordant in time with appearance of MHC class II E beta mRNA and Ia invariant chain mRNA. The increase in cathepsin H mRNA levels by IFN-gamma was dose dependent. Cycloheximide treatment of peritoneal macrophages inhibited the increase in cathepsin H mRNA levels induced by IFN-gamma, suggesting that the increase in cathepsin mRNA levels requires de novo protein synthesis. Lipopolysaccharide and cytokines interleukin-2 (IL-2), IL-4, IL-10, and tumor necrosis factor alpha were found to have no effect on cathepsin H mRNA levels in mouse peritoneal macrophages. |
