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Publication : Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq.

First Author  Petry F Year  1989
Journal  FEBS Lett Volume  258
Issue  1 Pages  89-93
PubMed ID  2591537 Mgi Jnum  J:10130
Mgi Id  MGI:58586 Doi  10.1016/0014-5793(89)81622-9
Citation  Petry F, et al. (1989) Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq. FEBS Lett 258(1):89-93
abstractText  cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.
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