| First Author | Hattori M | Year | 2000 |
| Journal | Science | Volume | 289 |
| Issue | 5483 | Pages | 1360-5 |
| PubMed ID | 10958785 | Mgi Jnum | J:64122 |
| Mgi Id | MGI:1888769 | Doi | 10.1126/science.289.5483.1360 |
| Citation | Hattori M, et al. (2000) Regulated cleavage of a contact-mediated axon repellent [see comments] [comment]. Science 289(5483):1360-5 |
| abstractText | Contact-mediated axon repulsion by ephrins raises an unresolved question: these cell surface ligands form a high-affinity multivalent complex with their receptors present on axons, yet rather than being bound, axons can be rapidly repelled. We show here that ephrin-A2 forms a stable complex with the metalloprotease Kuzbanian, involving interactions outside the cleavage region and the protease domain. Eph receptor binding triggered ephrin-A2 cleavage in a localized reaction specific to the cognate ligand. A cleavage-inhibiting mutation in ephrin-A2 delayed axon withdrawal. These studies reveal mechanisms for protease recognition and control of cell surface proteins, and, for ephrin-A2, they may provide a means for efficient axon detachment and termination of signaling. |
