| First Author | Mayer C | Year | 2008 |
| Journal | EMBO Rep | Volume | 9 |
| Issue | 8 | Pages | 774-80 |
| PubMed ID | 18600236 | Mgi Jnum | J:150911 |
| Mgi Id | MGI:3852298 | Doi | 10.1038/embor.2008.109 |
| Citation | Mayer C, et al. (2008) The structure of NoRC-associated RNA is crucial for targeting the chromatin remodelling complex NoRC to the nucleolus. EMBO Rep 9(8):774-80 |
| abstractText | Silencing of ribosomal RNA genes (rDNA) requires binding of the chromatin remodelling complex NoRC to RNA that is complementary to the rDNA promoter. NoRC-associated RNA (pRNA) folds into a conserved stem-loop structure that is required for nucleolar localization and rDNA silencing. Mutations that disrupt the stem-loop structure impair binding of TIP5, the large subunit of NoRC, to pRNA and abolish targeting of NoRC to nucleoli. Binding to pRNA results in a conformational change of TIP5, as shown by enhanced sensitivity of TIP5 towards trypsin digestion. Our results indicate an RNA-dependent mechanism that targets NoRC to chromatin and facilitates the interaction with co-repressors that promote heterochromatin formation and rDNA silencing. |
