| First Author | Jenne DE | Year | 1991 |
| Journal | J Immunol | Volume | 147 |
| Issue | 3 | Pages | 1045-52 |
| PubMed ID | 1861068 | Mgi Jnum | J:11357 |
| Mgi Id | MGI:59793 | Doi | 10.4049/jimmunol.147.3.1045 |
| Citation | Jenne DE, et al. (1991) Genomic organization and subchromosomal in situ localization of the murine granzyme F, a serine protease expressed in CD8+ T cells. J Immunol 147(3):1045-52 |
| abstractText | Granzyme F belongs to a closely related family of seven murine serine proteases stored in cytoplasmic granules of lymphoid cell populations. In contrast to the murine granzymes A to E and G, granzyme F is exclusively expressed in the CD4-CD8+ subset of peripheral T cells. To characterize the genomic sequences responsible for its highly restricted expression, we isolated a cosmid clone and sequenced a 7.5-kb genomic fragment that contains the promoter region and all five exons of the murine granzyme F gene. A TATA box sequence is located at position -25 relative to the transcription initiation site, which was determined by RNase protection. The genomic organization of granzyme F is similar to that of granzyme B and granzyme C, leukocyte elastase, cathepsin G, rat mast cell protease II, and complement factor D (adipsin). By the use of two fluorochromes for simultaneous high resolution in situ hybridization, the granzyme F gene was localized in close proximity distally from the TCR alpha-chain locus on mouse chromosome 14. |
