| First Author | Ohno T | Year | 2007 |
| Journal | Biochem Biophys Res Commun | Volume | 354 |
| Issue | 4 | Pages | 1010-5 |
| PubMed ID | 17274955 | Mgi Jnum | J:120341 |
| Mgi Id | MGI:3706293 | Doi | 10.1016/j.bbrc.2007.01.084 |
| Citation | Ohno T, et al. (2007) The negative transcription factor E4BP4 is associated with circadian clock protein PERIOD2. Biochem Biophys Res Commun 354(4):1010-5 |
| abstractText | The bZIP transcription factor E4BP4, is a mammalian homologue of vrille that functions as a key negative component of the circadian clock. We have shown that the E4BP4-binding site (B-site) is required in addition to a non-canonical E-box (E2 enhancer) for robust circadian Period2 (Per2) expression in the cell-autonomous clock. While the E2 enhancer and the B-site are closely situated, correlations between each component bound to the E2 enhancer and the B-site remain obscure. Here, we show that E4BP4 interacts with PER2, which represses transcriptional activity via the E-box enhancer. Interaction with PER2 required the carboxyl-terminal region that contains the repression domain of E4BP4. We also found that E4BP4 interacts with CRYPTOCHROME2 (CRY2), a key negative regulator in the mammalian circadian clock. These results suggest that E4BP4 is a component of the negative regulator complex of mammalian circadian clocks. |
