| First Author | Hanna MC | Year | 2000 |
| Journal | J Neurochem | Volume | 75 |
| Issue | 1 | Pages | 240-7 |
| PubMed ID | 10854267 | Mgi Jnum | J:62799 |
| Mgi Id | MGI:1859688 | Doi | 10.1046/j.1471-4159.2000.0750240.x |
| Citation | Hanna MC, et al. (2000) Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents. J Neurochem 75(1):240-7 |
| abstractText | The gene and cDNAs that encode a novel subunit of rodent serotonin 5-HT(3) receptors were isolated from mouse and rat tissues. Each of the new rodent subunits shares 40% amino acid identity with the rat 5-HT(3A) subunit and 73% identity with the human 5-HT(3B) subunit. Despite a relatively low level of structural conservation, sequence analysis and functional studies suggest that the new rodent subunits are orthologues of the human 5-HT(3B) subunit. In common with homologous human receptors, rat heteromeric 5-HT(3) receptors displayed a substantially larger single-channel conductance than homomeric 5-HT(3A) receptors. In addition, the rat heteromeric receptors were less sensitive to antagonism by tubocurarine. However, in contrast to human heteromeric receptors, those of the rat displayed pronounced inward rectification of both the whole-cell and single-channel current amplitudes. Transcripts of the mouse 5-HT(3A) and 5-HT(3B) subunits are coexpressed in several cell lines that possess endogenous 5-HT(3) receptors. In addition, treatment of rat PC12 cells with nerve growth factor induced expression of both subunit mRNAs, with a similar time course for accumulation of each transcript. The combination of functional data and expression patterns is consistent with the existence of heteromeric 5-HT(3) receptors in rodent neurons. |
