| First Author | Kashiwamura S | Year | 1990 |
| Journal | J Immunol | Volume | 145 |
| Issue | 1 | Pages | 337-43 |
| PubMed ID | 2358676 | Mgi Jnum | J:10573 |
| Mgi Id | MGI:59021 | Doi | 10.4049/jimmunol.145.1.337 |
| Citation | Kashiwamura S, et al. (1990) Structure of the murine mb-1 gene encoding a putative sIgM-associated molecule. J Immunol 145(1):337-43 |
| abstractText | Genomic DNA clones containing the B cell-specific murine mb-1 gene were isolated and a 5.6-kb BamH I fragment was characterized. It is 5629 bp long and contains five exons: an exon containing the 5' untranslated and the coding sequence of the signal peptide, an exon of 294 bp, which contains most of the extracellular sequence of the MB-1 protein, a 119-bp long exon coding mainly for the transmembrane portion, and two exons of 69 bp and 427 bp encoding the cytoplasmic domain and the 3'-untranslated region, respectively. The mb-1 gene does not contain a TATA box found in many eukaryotic promoters. The 5'-flanking region has sequence stretches homologous to IgVH 5'-promoter regions and a bcl 2 intron sequence. It contains the decanucleotide sequence (ATGGCAAATA) almost identical to the octamer motif of IgVH promoters. A B cell-specific DNase I-hypersensitive site was found in the 3'-flanking region indicating that this region might be involved in B cell-specific expression of mb-1. Southern blot analysis of genomic liver DNA with the cloned mb-1 cDNA suggests the existence of another mb-1-related gene segment. |
