| First Author | Gorman SD | Year | 1988 |
| Journal | J Immunol | Volume | 140 |
| Issue | 10 | Pages | 3646-53 |
| PubMed ID | 3258885 | Mgi Jnum | J:9152 |
| Mgi Id | MGI:57615 | Doi | 10.4049/jimmunol.140.10.3646 |
| Citation | Gorman SD, et al. (1988) Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression. J Immunol 140(10):3646-53 |
| abstractText | We have isolated the Ly-3 gene by chromosomal walking from the previously cloned Ly-2 gene. These genes are shown to be 36 kb apart and in the same transcriptional orientation. Transfection of the Ly-3 gene into mouse L cells results in cell surface expression of Ly-3 protein only in the presence of Ly-2 (or its human homolog, CD8), although Ly-2 surface expression is not similarly dependent on Ly-3. cDNA clones encoding Ly-3 have been isolated and sequenced and show little sequence similarity to Ly-2, whereas both Ly-2 and Ly-3 are homologous to Ig variable regions. One cDNA clone encodes a form of Ly-3 lacking a transmembrane region. Although two alleles of Ly-3 have been previously defined serologically, Northern blot analyses of Ly-3 mRNA from a series of inbred mouse strains show unusual polymorphisms in the lengths of Ly-3 mRNA species defining at least three allelic variants of this gene. |
