| First Author | Jimenez-Lara AM | Year | 2000 |
| Journal | FEBS Lett | Volume | 468 |
| Issue | 2-3 | Pages | 203-10 |
| PubMed ID | 10692587 | Mgi Jnum | J:69173 |
| Mgi Id | MGI:1934160 | Doi | 10.1016/s0014-5793(00)01209-6 |
| Citation | Jimenez-Lara AM, et al. (2000) Cloning of a mouse glucocorticoid modulatory element binding protein, a new member of the KDWK family. FEBS Lett 468(2-3):203-10 |
| abstractText | A mouse cDNA that encodes a nuclear DNA binding protein was identified by yeast two-hybrid screening using the activation domain 2 of the nuclear receptor coactivator TIF2 as a bait. BLAST analysis revealed that the identified cDNA encodes a KDWK domain and contains sequences almost identical to three tryptic peptides of rat GMEB-1 which together with the GMEB-2 heterodimeric partner binds to the GME/CRE sequence (glucocorticoid modulatory element) of the tyrosine aminotransferase (TAT) promoter. Mouse GMEB-1 is ubiquitously expressed in all the tissues examined. In vitro translated mGMEB-1 bound specifically to GME oligonucleotides, either alone or as a heterodimer with rGMEB-2. Transient transfection experiments with TAT promoter reporter genes suggest a potential role for mGMEB-1 as a transcriptional regulator of the TAT promoter. |
