| First Author | Shiratsuchi G | Year | 2015 |
| Journal | EMBO J | Volume | 34 |
| Issue | 1 | Pages | 97-114 |
| PubMed ID | 25385835 | Mgi Jnum | J:233666 |
| Mgi Id | MGI:5787766 | Doi | 10.15252/embj.201488979 |
| Citation | Shiratsuchi G, et al. (2015) RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34(1):97-114 |
| abstractText | Formation of a new centriole adjacent to a pre-existing centriole occurs only once per cell cycle. Despite being crucial for genome integrity, the mechanisms controlling centriole biogenesis remain elusive. Here, we identify RBM14 as a novel suppressor of assembly of centriolar protein complexes. Depletion of RBM14 in human cells induces ectopic formation of centriolar protein complexes through function of the STIL/CPAP complex. Intriguingly, the formation of such structures seems not to require the cartwheel structure that normally acts as a scaffold for centriole formation, whereas they can retain pericentriolar material and microtubule nucleation activity. Moreover, we find that, upon RBM14 depletion, a part of the ectopic centriolar protein complexes in turn assemble into structures more akin to centrioles, presumably by incorporating HsSAS-6, a cartwheel component, and cause multipolar spindle formation. We further demonstrate that such structures assemble in the cytoplasm even in the presence of pre-existing centrioles. This study sheds light on the possibility that ectopic formation of aberrant structures related to centrioles may contribute to genome instability and tumorigenesis. |
