| First Author | Lancman JJ | Year | 2005 |
| Journal | Dev Dyn | Volume | 234 |
| Issue | 4 | Pages | 948-60 |
| PubMed ID | 16245339 | Mgi Jnum | J:102854 |
| Mgi Id | MGI:3608165 | Doi | 10.1002/dvdy.20591 |
| Citation | Lancman JJ, et al. (2005) Analysis of the regulation of lin-41 during chick and mouse limb development. Dev Dyn 234(4):948-60 |
| abstractText | We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo. |
