| First Author | Ozawa M | Year | 1996 |
| Journal | J Biochem | Volume | 119 |
| Issue | 2 | Pages | 302-8 |
| PubMed ID | 8882722 | Mgi Jnum | J:31902 |
| Mgi Id | MGI:79408 | Doi | 10.1093/oxfordjournals.jbchem.a021239 |
| Citation | Ozawa M, et al. (1996) Molecular cloning and expression of a mouse alpha-1,3 fucosyltransferase gene that shows homology with the human alpha-1,3 fucosyltransferase IV gene. J Biochem 119(2):302-8 |
| abstractText | The polymerase chain reaction was used to amplify and clone a fragment of a novel fucosyltransferase cDNA from mouse embryonal carcinoma F9 cells. Southern blot analysis of mouse genomic DNA with the cloned cDNA fragment revealed that there is a single copy of the fucosyltransferase gene per haploid genome. A 5 kb EcoRI restriction fragment of the genomic DNA hybridized with the cDNA has been cloned and a 1,697 bp fragment containing the sequence of the cDNA was sequenced. The nucleotide sequence and the deduced amino acid sequence of the clone showed a highest degree of homology with those of human alpha-1,3 fucosyltransferase IV, i.e. 77.1 and 75.5% identity, respectively. Consistent with the sequence similarity, the transfection of a 1,544 bp fragment under the control of the cytomegarovirus enhancer and beta actin promoter into COS-1 cells confers alpha-1,3 fucosyltransferase activity on the cells, resulting in cell surface expression of SSEA-1. |
