| First Author | Gubitz AK | Year | 1999 |
| Journal | Genomics | Volume | 55 |
| Issue | 2 | Pages | 248-51 |
| PubMed ID | 9933574 | Mgi Jnum | J:52873 |
| Mgi Id | MGI:1330536 | Doi | 10.1006/geno.1998.5661 |
| Citation | Gubitz AK, et al. (1999) Assignment of the melatonin-related receptor to human chromosome X (GPR50) and mouse chromosome X (Gpr50). Genomics 55(2):248-51 |
| abstractText | Recent efforts to clone further members of the melatonin receptor family have led to the identification of a novel G-protein-coupled receptor in human pituitary. This receptor, referred to as H9, is clearly related to high- affinity melatonin receptors yet unable to bind this hormone. We now report the cloning and expression of the cDNA encoding the H9 receptor in mice. The mouse clone encodes a protein of 591 amino acids that shares 74% amino acid identity with the human receptor and is unable to bind 2-[I-125]iodomelatonin when transiently expressed in COS-7 cells. We also determined the chromosome loci of the human and mouse H9 receptor genes. Both genes were found to be X-linked: radiation hybrid mapping revealed that the human H9 gene (GPR50) is localized to Xq28. The mouse gene (Gpr50) was determined to lie in the proximal portion of chromosome X by means of interspecific backcross analysis. These loci might be relevant to genetically based neuroendocrine disorders. (C) 1999 Academic Press. |
