| First Author | Kitajima K | Year | 1999 |
| Journal | Dev Dyn | Volume | 215 |
| Issue | 3 | Pages | 206-14 |
| PubMed ID | 10398531 | Mgi Jnum | J:56108 |
| Mgi Id | MGI:1340104 | Doi | 10.1002/(SICI)1097-0177(199907)215:3<206::AID-AJA3>3.0.CO;2-X |
| Citation | Kitajima K, et al. (1999) Expression of a novel type of classic cadherin, PB-cadherin in developing brain and limb buds. Dev Dyn 215(3):206-14 |
| abstractText | PB-cadherin is a novel classic type of cadherin predominantly expressed in brain of adult rats (Sugimoto et al. [1996] J. Biol. Chem. 271:11548-11556). To examine the spatial and temporal expression of PB-cadherin during development, we isolated full-length cDNA of mouse PB-cadherin and studied its expression pattern in mouse embryos. In Northern blots, PB-cadherin mRNA was detected at 9.5 days postcoitum (dpc) onwards. Whole-mount in situ hybridization showed that PB-cadherin signals mainly occurred in developing neural tissues, including brain and spinal cord, and limb buds in the 10.5 dpc embryo. In the brain, PB-cadherin mRNA were strongly expressed in the forebrain and midbrain-hindbrain boundary region (isthmus). In isthmus, PB-cadherin expression delineated the expression area of Wnt-1, a secreted signaling molecule essential for proper cerebellum development. In the developing limb, PB-cadherin mRNA was first localized in posterior part of buds at 10.5 dpc, and was thereafter distributed in a domain around the digit rudiments. This expression pattern is similar to that of BMP-2, a secreted signalling molecule involving limb patterning and morphogenesis. These findings suggested the possibility that PB-cadherin-mediated cell-cell adhesion has a functional role in pattern formation and morphogenesis of mouse embryonic brain and limb. Dev Dyn 1999;215:206-214. Copyright 1999 Wiley-Liss, Inc. |
