| First Author | Moschella MC | Year | 1999 |
| Journal | Gene Expr | Volume | 8 |
| Issue | 1 | Pages | 59-66 |
| PubMed ID | 10543731 | Mgi Jnum | J:60099 |
| Mgi Id | MGI:1352765 | Citation | Moschella MC, et al. (1999) SM-20 is a novel growth factor-responsive gene regulated during skeletal muscle development and differentiation. Gene Expr 8(1):59-66 |
| abstractText | SM-20 is a novel, evolutionarily conserved 'early response' gene originally cloned from a rat aortic smooth muscle cell (SMC) cDNA library. SM-20 encodes a cytoplasmic protein, which is induced by platelet-derived growth factor and angiotensin II in cultured SMC and is upregulated in intimal SMC of atherosclerotic plaques and injured arteries. We have now examined SM-20 expression during differentiation of cultured skeletal myoblasts and during skeletal myogenesis in vivo. Low levels of SM-20 mRNA and protein were expressed in proliferating mouse C2C12 myoblasts. Differentiation by serum withdrawal was associated with a marked induction of SM-20 mRNA and the expression of high levels of SM-20 antigen in myotubes. The induction was partially inhibited by blocking differentiation with bFGF or TGFbeta. Similar results were obtained with the nonfusing mouse C25 myoblast line, suggesting that SM-20 upregulation is a consequence of biochemical differentiation and is fusion independent. During mouse embryogenesis, SM-20 was first observed at 8.5E in the dermomyotomal cells of the rostral somites. SM-20 expression progressed in a rostral to caudal pattern, with highest levels seen in the muscle primordia and mature muscles. SM-20 thus represents a novel intracellular protein that is regulated during skeletal muscle differentiation and development. |
