| First Author | Shipman PM | Year | 1988 |
| Journal | J Cell Biochem | Volume | 38 |
| Issue | 3 | Pages | 189-98 |
| PubMed ID | 2906640 | Mgi Jnum | J:22452 |
| Mgi Id | MGI:70324 | Doi | 10.1002/jcb.240380306 |
| Citation | Shipman PM, et al. (1988) Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes. J Cell Biochem 38(3):189-98 |
| abstractText | Expression of cyclin, a non-histone nuclear protein, during recombinant interleukin 2 (rIL2)-driven cell-cycle progression of cloned T lymphocytes has been assessed. We found that expression of cyclin protein, as detected by immunofluorescence, is tightly associated with proliferation, and not merely S-phase, of L2 cells stimulated with rIL2. Cyclin immunofluorescence was detected in all cell-cycle phases (G1/S/G2/M, as detected by flow cytometry) of proliferating L2 cells. Accumulation of cyclin mRNA levels was induced as early as 1 h after stimulation, was maximal at 25-49 h, and remained elevated throughout stimulation, as detected by Northern blot analysis. A cDNA-encoding murine cyclin was cloned from a cDNA library prepared from IL2-stimulated cloned T cells. The sequence of the 5' end of the murine cyclin cDNA was determined and found to be 88% and 82% similar to the sequences of cDNA clones encoding rat and human cyclin, respectively. The present studies demonstrate that cyclin protein and mRNA accumulation are highly regulated during IL2-induced proliferation of a cloned T cell. These data provide a framework for addressing the molecular mechanisms regulating cyclin gene expression during cellular proliferation. |
