| First Author | Vaux DL | Year | 1995 |
| Journal | DNA Cell Biol | Volume | 14 |
| Issue | 3 | Pages | 189-93 |
| PubMed ID | 7880439 | Mgi Jnum | J:23943 |
| Mgi Id | MGI:71727 | Doi | 10.1089/dna.1995.14.189 |
| Citation | Vaux DL, et al. (1995) Cloning of mouse RP-8 cDNA and its expression during apoptosis of lymphoid and myeloid cells. DNA Cell Biol 14(3):189-93 |
| abstractText | A partial-length cDNA encoding a cell death-associated gene designated RP-8 was cloned from rat thymocytes by Owens and co-workers (Mol. Cell. Biol. 11, 4177-4188, 1991). They reported that transcription of RP-8 was induced when rat thymocytes were caused to undergo apoptosis triggered by either radiation or treatment with dexamethasone. To study the role of RP-8 in cell death in the mouse, we have cloned a full-length mouse RP-8 cDNA from a mouse thymus cDNA library. The mouse RP-8 gene encodes of protein of 343 amino acids with 92% amino acid identity to the rat RP-8 protein. Expression of mouse RP-8 was not altered in a factor-dependent myeloid line induced to undergo apoptosis by growth factor withdrawal, or when a lymphoid cell line was triggered to undergo apoptosis by irradiation. Although these data do not prove that RP-8 is not a cell death gene, they show that RP-8 expression is not sufficient for apoptosis, and that transcriptional up regulation of RP-8 is not universally associated with apoptosis. |
